Bioline Scholar Monthly: April 2012 Roundup – Breast Cancer

Focus on Breast Cancer

For years scientists have been treating breast cancer as a single disease. However, a new landmark study published in Nature has reclassified breast cancer into ten separate sub-diseases based on their genetic fingerprint. The culmination of decades of research, the study is the largest global study of breast cancer tissue ever performed.

The team, led by the British Columbia Cancer Center in Canada and the Cambridge Cancer Research Institute in the UK, used genome-wide microarrays to analyze the DNA and RNA of 2,000 tumor samples taken from women diagnosed with breast cancer. This huge pool of genetic information (copy number variants, SNPs and gene expression data), as well as survival data, allowed researchers to spot new and previously unacknowledged patterns for ten subtly different cancers that have, historically, been considered as one.

The challenge now is to understand the genetic drivers behind these newly discovered breast cancer variants and to develop new targeted therapies in the future. It could also lead to women with the best prognosis being spared side-effects of chemotherapy. The classification system will likely also form the basis for newer and better ways to diagnose and manage the disease.

Bioline offers a number of reagents that have helped further the study of cancers and, more specifically, breast cancer. So this edition of Bioline Scholar Monthly focuses on the use of Bioline reagents and kits in the field of breast cancer research.

SensiMix SYBR & Fluorescein Kit
In a diverse cohort of breast cancer patients with a 1–5 year tumor relapse versus those with up to 7 years relapse-free survival, RNA was extracted and subjected to microarray and real-time RT-PCR analysis. Among the 299 genes, five genes which included B cell response genes were found to predict with >85% accuracy relapse-free survival. Real-time RT-PCR confirmed the 5-gene prognostic signature that was distinct from an FDA-cleared 70-gene signature of MammaPrint panel and from the Oncotype DX recurrence score assay panel.

Ascierto, L. M., et al. Breast Can. Res. Treat. 131(3):871-880 (2012) – A signature of immune function genes associated with recurrence-free survival in breast cancer patients.

TRIsure reagent
MicroRNAs (miRNAs) are noncoding RNAs that function as key posttranscriptional regulators of gene expression. This paper found that BRCA1 recognizes the RNA secondary structure and directly binds with primary transcripts of miRNAs via a DNA-binding domain. The findings indicate novel functions of BRCA1 in miRNA biogenesis, which may be linked to its tumor suppressor mechanism and maintenance of genomic stability.

Kawai S. and Amano A. J. Cell Biol. 197 (2):201-208 (2012) – BRCA1 regulates microRNA biogenesis via the DROSHA microprocessor complex.

Bioscript
The bioactive lipid sphingosine 1-phosphate (S1P) uses sphingosine 1-phosphate receptor 4 (S1P4) and human epidermal growth factor receptor 2 (HER2) to stimulate the extracellular signal regulated protein kinase 1/2 (ERK-1/2) pathway in MDA-MB-453 cells. The magnitude of the signaling gain on the ERK-1/2 pathway produced in response to S1P can be increased by HER2 in MDA-MB-453 cells. The linkage of S1P with an oncogene suggests that S1P and specifically S1P4 may have an important role in breast cancer progression.

Long J. S., et al. J. Biol. Chem. 285:35957-35966 (2010) – Sphingosine 1-Phosphate Receptor 4 Uses HER2 (ERBB2) to Regulate Extracellular Signal Regulated Kinase-1/2 in MDA-MB-453 Breast Cancer Cells.

SensiMix SYBR No-ROX Kit
CD44, the transmembrane receptor for hyaluronan, is implicated in tumor cell invasion and metastasis. The expression of CD44 and its variants is associated with poor prognosis in breast cancer. This paper investigated the effect of silibinin (a polyphenolic flavonolignan of the herbal plant of Silybum marianum, milk thistle) on the epidermal growth factor (EGF) ligand-induced CD44 expression in human breast cancer cells. The results suggest that silibinin prevents the EGFR signaling pathway and may be used as an effective drug for the inhibition of metastasis of human breast cancer.

Kim S., et al. Anticancer Res. 31(11): 3767-3773 (2011) – Silibinin Suppresses EGFR Ligand-induced CD44 Expression through Inhibition of EGFR Activity in Breast Cancer Cells.

Human papillomavirus (HPV) and Epstein Barr virus (EBV) have been found in breast carcinomas around the world. In this study, fifty-five BCs from Chile were analyzed for HPV and EBV presence. In addition, HPV- 16 viral load/physical status and E6/E7 expressions were determined. The results suggest that it is unlikely that HPV and/or EBV play a direct role in the etiology of breast carcinomas.

Aguayo F., et al. Infectious Agents and Cancer 6:7 (2011) – Human papillomavirus and Epstein-Barr virus infections in breast cancer from chile.

cDNA Synthesis Kit
This study suggests that melatonin may play a role in the desmoplastic reaction in breast cancer through a down regulatory action on the expression of antiadipogenic cytokines, which decrease the levels of these cytokines. Lower levels of cytokines stimulate the differentiation of fibroblasts and decrease both aromatase activity and expression, thereby reducing the number of estrogen-producing cells proximal to malignant cells.

Alonso-González C., et al. J. Pineal Res. 52(3): 282–290, (2012) – Melatonin interferes in the desmoplastic reaction in breast cancer by regulating cytokine production.

Melatonin reduces the development of breast cancer interfering with oestrogen-signalling pathways, and also inhibits aromatase activity and expression. This study shows that melatonin inhibits aromatase activity and expression by regulating the gene expression of specific aromatase promoter regions. A possible mechanism for these effects would be the regulation by melatonin of intracellular cAMP levels, mediated by an inhibition of cyclooxygenase activity and expression.

Martínez-Campa C., et al. British J. Can. 101: 1613–1619 (2009) – Melatonin inhibits aromatase promoter expression by regulating cyclooxygenases expression and activity in breast cancer cells.

IMMOLASE DNA Polymerase
Bisphenol A (BPA) has long been suspected to promote carcinogenesis, but the high doses of BPA used in many studies generated conflicting results. This paper shows that BPA at environmentally relevant doses reduces the efficacy of chemotherapeutic agents. These data provide considerable support to the accumulating evidence that BPA is hazardous to human health.

LaPensee E. W., et al. Environ Health Perspect. 117(2): 175–180 (2009) – Bisphenol A at Low Nanomolar Doses Confers Chemoresistance in Estrogen Receptor-a–Positive and –Negative Breast Cancer Cells.

BIOTAQ DNA Polymerase
This paper indicates that decreased CDH1, CDH13 and TIMP3 with increased CD44 gene expression levels can be used as an indicator for invasion in both ER-positive and ER-negative breast tumors. In double-negative tumor tissues, CD44 can be considered a marker for aggressive properties. However, additional assays in a larger series of patients with long follow up will be necessary to confirm these results of gene expressions in ER-positive and ER-negative tumors and their relationship with HER2 and ESR1.

Celebiler A., et al. Can. Sci. 100: 2341–2345. (2009) – Predicting invasive phenotype with CDH1, CDH13, CD44, and TIMP3 gene expression in primary breast cancer.

Bioline Scholar Monthly: March 2012 Roundup – Epigenetics

Focus on Epigenetics

Epigenetics is the study of heritable changes in gene expression or cellular phenotype caused by mechanisms other than changes in the underlying DNA sequence. The term refers to functionally relevant modifications to the genome that do not involve a change in the nucleotide sequence. Examples of such changes are DNA methylation and histone modification, both of which serve to regulate gene expression without altering the underlying DNA sequence.

These changes may remain through cell divisions for the remainder of the cell’s life and may also last for multiple generations. However, there is no change in the underlying DNA sequence of the organism; instead, non-genetic factors cause the organism’s genes to behave differently.

Just as in genetic changes, epigenetics is known to be involved in diseases such as cancer, silencing control genes. Epigenetics can also be beneficial by helping control gene expression during development and silence viral insertions, preventing them from promoting viral proliferation. Scientists have only just started scratching the surface of epigenomes, trying to make sense of the patterns of epigenetic marks.

Bioline has a number products designed to help in these studies and this edition of Bioline Scholar Monthly focuses on the use of Bioline products in the developing field of epigenetics research.

5-hydroxymethyl-dCTP
Due to their very similar chemical structure, discrimination of the rare hmC against the far more abundant mC is technically challenging and to date no methods for direct sequencing of hmC have been reported. This paper used 5-hydroxymethyl-dCTP to report on a purified recombinant endonuclease, PvuRts1I, which selectively cleaves hmC-containing sequences, showing its potential to interrogate hmC patterns in mammalian genomes.

Szwagierczak, A., et al. Nucl. Acids Res. 39(12): 5149-5156. (2011) – Characterization of PvuRts1I endonuclease as a tool to investigate genomic 5–hydroxymethylcytosine

Model substrates were created using 5-hydroxymethyl-dCTP to show its effects, both at the promoter and in the gene body, on in vitro gene transcription. The results suggest that the presence of 5hmC in a promoter prevents the binding of essential transcription factors or recruits factors that repress transcription.

Robertson, J., et al. Biochem. Biophy. Res. Comm. 411(1): 40–43 (2011) – The presence of 5-hydroxymethylcytosine at the gene promoter and not in the gene body negatively regulates gene expression

Bioline’s dCTP and 5-hydroxymethyl-dCTP were used to help determined hmC levels in various adult tissues and differentiating embryonic stem cells and show a correlation with differential expression of tet genes.

Szwagierczak, A., et al. Nucl. Acids Res. 38(19): e181. (2010) – Sensitive enzymatic quantification of 5-hydroxymethylcytosine in genomic DNA

SensiFAST™ SYBR Lo-ROX Kit
RT-qPCR was performed using SensiFAST SYBR Lo-ROX on primary cultures and ovarian cell lines for SFRP4 and its key downstream regulators. The results support a role for SFRP4 as a tumor suppressor gene in ovarian cancers via inhibition of the Wnt signaling pathway. This has not only predictive implications but could also facilitate a therapeutic role using epigenetic targets.

Jacob, F., et al. PLoS ONE 7(2):e31885.doi:10.1371/journal.pone.0031885 (2012) – Loss of Secreted Frizzled-Related Protein 4 Correlates with an Aggressive Phenotype and Predicts Poor Outcome in Ovarian Cancer Patients

IMMOLASE™ DNA polymerase
Radical surgery is the de facto treatment for early rectal cancer. Conservative surgery can achieve high rates of cure but the histopathological measures of outcome used to select local treatment lack precision. In this paper five sites were significantly hypermethylated in cancer compared with adjacent tissue and hypermethylation of two or more of these genes was associated with localized disease.

Leong, K. J., et al. Br. J. Surg, 98: 724–734 (2011) – Methylation profiling of rectal cancer identifies novel markers of early-stage disease

The Sry (sex determining region on Y chromosome) gene is a master gene for sex determination. The Sry gene has tissue-dependent and differentially methylated regions. This study found unique non-CpG methylation in the Sry T-DMR. This non-CpG methylation was detected several mouse strains and has been associated with gene expression in the developmental process. The finding shows that non-CpG methylation has unique characteristic and is still conserved in mammals.

Nishino, K., et al. J. Reprod. Develop. 57(5): 586-593 (2011) – Non-CpG Methylation Occurs in the Regulatory Region of the Sry Gene

MangoTaq™ DNA Polymerase
Orchestrated interplay of different epigenetic mechanisms in regulating gene expression throughout development, shedding light on the current hypotheses for the origin and mechanism of imprinting in plant endosperm.

Paterson, S., et al DMD 39(1): 77-82 (2011) – Histone Deacetylase Inhibitors Increase Human Arylamine N-Acetyltransferase-1 (NAT1) Expression in Human Tumor Cells

Over 90% of low risk (LR) neuroblastoma patients survive whereas less than 30% of high risk (HR) patients are long term survivors. Age (children younger than 18 months old) is associated with LR disease. This paper suggests that adaptive immune responses may play an important role in the progression of HR disease whereas innate immune responses may be active in LR patients.

Gowda, M., et al. J. Tran. Med. 9:170 (2011) – Distinct signatures of the immune responses in low risk versus high risk neuroblastoma

TRIsure™
Genetic engineering can expand the utility of pigs for modeling human diseases, however the inefficient production of transgenic pigs represents a technological bottleneck. This paper assesses the hyperactive Sleeping Beauty transposon system for transgene integration into the embryonic porcine genome. It demonstrates germ-line transmission, through F1-offspring and insertion into transposon-tagged genomic loci followed by nuclear transfer. This potentially facilitates the development of large animal models for human diseases.

Garrels, W., et al. Genome.PLoS ONE 6(8):e23573 (2011) – Germline Transgenic Pigs by Sleeping Beauty Transposition in Porcine Zygotes and Targeted Integration in the Pig

Bioline: The PCR Company is 20!

Bioline Celebrates 20 Years of Services to Life Science

How time flies! It’s 2012 and this year Bioline: The PCR Company celebrates its 20 year anniversary.

The world has changed rapidly and dramatically since Bioline was first established in 1992 by Marco Calzavara, president of Bioline, but we are still manufacturing and supplying a range of consistently high-quality products to life science researchers and institutions around the world.

From our base in London, UK, we have rapidly expanded to become a leading primary manufacturer and distributor of PCR enzymes and reagents for molecular biology; providing complete solutions to researchers in universities, major research institutions, hospitals, biotech firms, diagnostics and pharmaceutical firms and laboratories globally.

Bioline’s products have been cited thousands of times in leading journals by researchers all over the world and, as part of our 20 year anniversary celebrations, we’ve started to document some of these publications in our Bioline Scholar Monthly series of blog posts.

When asked about the milestone of two decades of service provision to life science, Marco Calzavara, founder and president of Bioline, commented:

“Our aim at Bioline for the past 20 years has been to develop high-quality and reliable products that really make the difference, delivering consistent results time after time. I am excited and proud to continue Bioline´s great work as a cutting edge molecular biology company, ensuring that we maintain our outstanding reputation as a high-quality and reliable firm.”

Richard L. Eberly, Chief Commercial Officer of Meridian Bioscience, Inc., added:

“Congratulations to the Bioline team on its 20th anniversary of successful operations throughout the world. We are enthusiastic about the success of the merger and impressed by the scientific capabilities of Bioline, as well as its well-deserved reputation for innovation and quality. We remain committed to expanding the rapidly growing portfolio of highly specialized molecular biology products from Bioline that enable the development of genomic tests utilized by researchers, clinical diagnostic laboratories, diagnostic test manufacturers and biotechnology companies.”

As part of the 20 year celebrations, we are running a monthly Mystery Prize Giveaway for the rest of 2012. Lucky customers will find a golden ticket hiding in their delivery, so keep a close look out when you receive your delivery because you may find one included with your next order!

We’d like all our customers over the years to join in the celebrations and you can do so by visiting the special Bioline 20 Year Anniversary microsite, where we have a timeline showing the major milestones over the last 20 years and an exclusive interview with our president, as well as other Bioline employees. You can also keep up to date with all the latest 20 year anniversary news and updates by following @ThePCRCompany and @BiolineUSA on Twitter.

It just remains to thank all our customers, both old and new alike, for your custom and we look forward to supplying you with more high-quality enzymes and reagents for the next 20 years!

Bioline Scholar Monthly: February 2012 Round-Up

It’s time for the second in our series of Bioline Scholar Monthly compilations. This month we’re focusing on the many uses of Bioline polymerases in forensic science.

Identification of a report’s species is one of the basic analyses in forensics. However, due to the nature of the sampling environment, DNA samples often contain PCR-inhibitory substances which may generate blank or incomplete DNA profiles. The common approach to overcoming PCR inhibition is extensive DNA purification, but this can increase the risk of DNA loss. In some cases, isolation of single cells using laser-capture microdissection can be used, but again this reduces the amount of DNA available.

DNA polymerases that can improve the quality of forensic DNA analysis and efficiently circumvent PCR inhibition, without any additional sample preparation, are therefore advantageous, as are polymerases that result in high yields.

Bioline’s DNA polymerases are very robust and have been carefully designed to overcome these problems. BIO-X-ACT™ Short in particular is specifically designed for difficult/problematic PCR applications that require high processivity and fidelity, applications that would normally fail with other DNA polymerase. MangoTaq™ has also been designed for problematic and ancient DNA, whereas IMMOLASE™ and BIOTAQ™ are high yield for small sample sizes. Together with our new MyTaq™, MyFi™ and RANGER Bioline has polymerases to meet all the high-fidelity requirements of forensic science.

So, without further ado, here’s…

Bioline Scholar Monthly: February 2012

BIO-X-ACT™ Short DNA Polymerase
Loop-mediated isothermal amplification (LAMP) is an uncomplicated, quick and relatively inexpensive diagnostic tool. In Barkway and colleagues procedure, BIO-X-ACT Short DNA Polymerase was initially used to successfully verify the LAMP primer pair for Eimeria species specificity using PCR.

Barkway, C. P., et al. BMC Veterinary Research 2011, 7:67 (2011) – Loop-mediated isothermal amplification (LAMP) assays for the species-specific detection of Eimeria that infect chickens

BIO-X-ACT™ Short Mix
In a comparison of nine DNA polymerases, the DNA detection limit was lowest with BIO-X-ACT Short, providing the highest number of improved DNA profiles, using real crime scene saliva samples.

Hedman, J., et al. BioTechniques 47, 951-958 (2009) – Improved forensic DNA analysis through the use of alternative DNA polymerases and statistical modeling of DNA profiles

IMMOLASE™ DNA Polymerase
The identification via DNA analysis is reliably and reproducibly possible from well preserved and semi-burnt bones.

Schwark, T., et al Forensic Sci. Int.: Gene. 5(5), 393-399 (2011) – Reliable genetic identification of burnt human remains.

Dried herbarium specimens may be invaluable to understand long-term changes at sites with a history of cyanobacterial blooms.

Metcalf, J.S., et al Harmful Algae 15 47–52 – (2012) – Analysis of microcystins and microcystin genes in 60–170-year-old dried herbarium specimens of cyanobacteria

MangoTaq™ DNA Polymerase
Out of 19 polymerases, the best performance was exhibited by the Mango-Taq DNA polymerase, which was the only polymerase which was able to amplify the ~620 bp amplification product from the 102 year old sample.

Telle, S. & Marco Thines, M. PLoS ONE 3(10):doi:10.1371/journal.pone.0003584 – (2008) – Amplification of cox2 (~620 bp) from 2 mg of Up to 129 Years Old Herbarium Specimens, Comparing 19 Extraction Methods and 15 Polymerases

BIOTAQ™ DNA Polymerase
In Australia and globally, Sarcophagidae flies remain unexploited as indicators of post-mortem interval in forensic investigations. A molecular identification method involving DNA ‘barcoding’ of the mitochondrial COI gene from 16 species of Australian Sarcophagidae was successfully developed. The authors conclude analysis of sarcophagids in forensic entomology should increase and their value as tools in criminal investigations realised.

Meiklejohn, K. A., et al. Int. J. Legal Med. 125(1), 27-32 – (2011) – DNA-based identification of forensically important Australian Sarcophagidae (Diptera)

BIOLASE™ DNA Polymerase
Microsatellite markers were developed for the medicinal plant Tripterygium (Celastraceae) to assess its population structure and to facilitate source tracking of plant materials used for medicinal extracts.

Novy, A. & Jones, K. C. Am. J. Bot. 98(10) e280-e281 (2011) – Characterization of polymorphic microsatellites for Tripterygium (Celastraceae), a monospecific genus of medicinal importance

BIOMIX™
Lindgren funnel traps baited with aggregation pheromones are effective tools for monitoring flight activity in the red flour beetle (Tribolium castaneum) and lesser grain borer (Rhyzopertha dominica). But the samples are affected by the preservation method.

Stevens, M. M,. et al. J. Stored Products Res. 47(2), 69–75 – (2011) – Maintaining DNA quality in stored-grain beetles caught in Lindgren funnel traps

BIOLINE Introduces SensiFAST™ HRM Kit for Gene Mutations & SNP Analysis

SensiFAST HRM Kit - your first choice for HRM curve analysis

Bioline is proud to announce the latest addition to its family of SensiFAST Real-Time PCR products.

SensiFAST™ HRM Kit facilitates High Resolution Melt (HRM) curve analysis, enabling amplification and discrimination of even the most challenging sequence differences -such as class 4 SNPs- without sequence preference.

SensiFAST HRM is designed to deliver fast, accurate detection of gene mutations and SNPs and provides reliable and highly reproducible data on all commonly used real-time PCR instruments, especially the new generation of fast-cyclers. SensiFAST HRM does not require expensive labelled oligonucleotide probes and offers a cost effective alternative to traditional probe based genotyping methods.

Marco Calzavara, President of Bioline said:

“I am delighted to announce the release of SensiFAST HRM as a new member of our real-time PCR-based SensiFAST family. This new kit will enable researchers to cost-effectively scan DNA from a variety of biological sample types to detect the smallest genetic variations among samples.”

For more information, please visit the SensiFAST™ HRM product page.

Show some love for Bioline, win an iPod Shuffle

Tell us why you like your favourite Bioline reagents in exchange for a free lab timer and a chance to win an iPod Shuffle

As no one can fail to have noticed by now, today is Valentine’s Day, a day when we all go out of our way to show special affection for the ones we love.

So, this February, why not show some love for Bioline by telling us why you use your favourite Bioline reagents? In return we’ll send you a free lab timer for your efforts.

But that’s not all! Because love is a two way thing, we’ve set aside some iPod Shuffles to give away to the originators of any product testimonials that we use.

We know that many scientists find Bioline’s high-quality reagents for molecular biology are the only ones that get the job done in-time, on-time, every time with their applications.

So, if this description fits you, you love your Bioline products and you’d like a free lab timer and a chance to win a 2GB iPod Shuffle, all you need to do is leave your testimonial as a comment and email us your contact details.

Or, if you prefer, email us directly with your quote and contact details and we’ll do the rest.

We look forward to hearing from you! In the meantime, Happy Valentine’s Day from all at Bioline: The PCR Company.

Introducing Bioline Scholar Monthly

Bioline Scholar Monthly - Focus on MyTaq™ DNA Polymerase Family

Keep up-to-date with cutting-edge developments in your particular area of research with Bioline Scholar Monthly, Bioline’s new resource for academic and industry researchers who want to stay ahead of the game.

Our team of scientists will be extracting the best in peer reviewed research and highlighting the different components that make up the diverse ecosystem of life sciences, from genetics to biochemistry, immunology, microbiology, neuroscience, cancer research, ecology, plant sciences and veterinary research.

Learn best practice from laboratory scientists around the world who have already published successfully with Bioline’s range of industry-leading products – designed to accelerate breakthroughs in all major fields of life science.

Thousands of papers cite Bioline products, so if you think we have missed an interesting peer-reviewed publication of yours, featuring any of our products, please get in touch by email, Twitter, Facebook, or leave a comment on the blog. You gain valuable exposure and profile for your group or company and we share your discoveries with the life science world.

Make an impact by connecting with Bioline Scholar Monthly today!

Features:

• Be first to hear about exciting, new, hot-off-the-press papers, novel applications and approaches from the global scientific community
• Discover highlights of the latest literature from all fields of life sciences featuring Bioline products
• Post-publication comments/interviews with researchers from the global Bioline community
• Tried and tested protocols from the literature to accelerate your research
• Pick up useful scientific hints, tips and tricks
• Comment and discuss – let us know what you think the latest findings means for your field

Bioline Scholar Monthly: January 2012 Round-Up

The first in our series of Bioline Scholar Monthly compilations focuses on the MyTaq™ DNA Polymerase Family.

MyTaq™ DNA Polymerase
Interesting set of papers for detecting the spread of novel genes from veterinary viruses.

Cadar, D., et al. Virus Genes doi: 10.1007/s11262-011-0650-4 (2011) – Genetic detection and analysis of porcine bocavirus type 1 (PoBoV1) in European wild boar (Sus scrofa)

Cadar, D., et al. Arch. Virol. doi: 10.1007/s00705-011-1125-6 (2011) – Distribution and genetic diversity of porcine hokovirus in wild boars

MyTaq™ Mix
Study of woodland population genetics by 454 shotgun pyrosequencing & PCR. Lead author of the paper, Dr Olivier Lepais, commented:

“We used MyTaq to screen for suitable microsatellite markers in simplex PCR. We found that MyTaq Mix gave great results and was very easy to work with. This ease of use was critical as the primer screening involved a high number of independent PCR mixes!”

Lepais, O. L. & Bacles, C. F. J. Hered. doi: 10.1093/jhered/esr062 (2011) – De Novo Discovery and Multiplexed Amplification of Microsatellite Markers for Black Alder (Alnus glutinosa) and Related Species Using SSR-Enriched Shotgun Pyrosequencing

MyTaq™ Red Mix
Study of microsatellite markers to study the genetic diversity of lobsters in Australia and New Zealand.

Thomas, L. & Bell, J.J. Conserv. Gene. Resources doi: 10.1007/s12686-011-9537-x (2011) – Characterization of polymorphic microsatellite markers for the red rock lobster, Jasus edwardsii (Hutton 1875)

MyTaq™ HS DNA Polymerase
Study on new molecular genetics assay for investigating harmful algal blooms, Sydney Institute of Marine Sciences.

Murray, S.A., et al. Appl. Envir. Microbiol. 77, 7050-7057(2011) – sxtA-Based Quantitative Molecular Assay To Identify Saxitoxin-Producing Harmful Algal Blooms in Marine Waters

MyTaq™ HS DNA Polymerase
Protocol on preparing ssDNA from PCR products with streptavidin magnetic beads.

Wilson, R. Nucleic Acid Therapeutics doi:10.1089/nat.2011.0322 (2011) – Preparation of Single-Stranded DNA from PCR Products with Streptavidin Magnetic Beads

MyTaq™ HS Mix
Interesting new veterinary parasitology paper from the University of New South Wales, Sydney & Merial, a Sanofi Company.

Šlapeta, J., et al. Vet Parasitol. doi: 10.1016/j.vetpar.2011.03.035 (2011) – The cat flea (Ctenocephalides f. felis) is the dominant flea on domestic dogs and cats in Australian veterinary practices

MyTaq™ HS Mix
Bacteria-triggered histone modification in a fungus showing previously undescribed evidence of Saga/Ada dependent histone acetylation triggered by prokaryotes.

Nützmann, H-W., et al. PNAS 108, 14282-14287 (2011) – Bacteria-induced natural product formation in the fungus Aspergillus nidulans requires Saga/Ada-mediated histone acetylation

MyTaq™ One Step RT-PCR Kit
Neuroscience paper from Department of Cell & Molecular Physiology, Neuroscience Center & Medical School at University of North Carolina.

Buttermore, E.D., et al. J. Neurosci. 31(22), 8013-8024 (2011) – The Cytoskeletal Adaptor Protein Band 4.1B Is Required for the Maintenance of Paranodal Axoglial Septate Junctions in Myelinated Axons